|PCR Optimization Guide|
|Reagent Selector Tool|
|Certificate of Analysis|
|qPCR Technical Support|
|PCR Technical Support|
|Q:||How much qARTA master mix do I need in my reaction?|
Details of the recommended master mix can be found in each of our User Protocols.
For a 5X mix and a 20uL reaction volume use 20 µL / 5 = 4 µL.
|Q:||Why don't qARTA qPCR mixes come in 2X?|
The 5X concentration is an integral part of being able to ship our products without ice to save transportation time and costs.
|Q:||My Cq value when using qARTA's EvaGreen mix is higher than when using my standard SYBRGreen mix?|
In melt curve analysis, qARTA EvaGreen mixes usually show a higher melting temperature because EvaGreen has a different chemistry than SYBRGreen. The Tm is usually 2°C higher.
|Q:||The qARTA PCR master mix had a low yield or no PCR product at all. Why?|
This may be due to a variety of reasons including: